![]() ![]() As a base to prepare special blood agar for Corynebacterium diphteriae, with cystine tellurite Y lamb's blood. As a base to prepare the classic blood agar that is used routinely in most laboratories. Trypticasein Soy Agar is used in the following cases: The prepared wedges are sown by surface area and serve to keep certain non-demanding microorganisms viable for a specified time. When they leave, they lean with the help of a support and let them solidify. Subsequently, the tubes are autoclaved at 121 ° C for 15 minutes. The agar preparation procedure is the same as that described for plates, with the difference that instead of serving the medium on Petri dishes, it is distributed between 10 to 12 ml in tubes with Bakelite covers before sterilizing. The color of the medium is chocolate brown. To prepare the TSA-based blood agar, proceed the same as the procedures already described, but when leaving the autoclave, let it rest until the temperature of the medium is approximately 56 to 70 ° C.At that time the blood is placed and mixed until the medium turns brown. Trypticasein soy agar plates supplemented with warmed blood The mixture is homogenized by rotating with gentle movements. Trypticasein soy agar plates supplemented with bloodīlood agar is prepared by adding 5% defibrinated blood at the time of cooling the tryptic soy agar to 50 ° C. Plates must reach room temperature before use. Prepared plates should be stored in a refrigerator (2-8 ° C) until use. You can also add 0.6% of yeast extract to favor the isolation of species of the genus Listeria, while the addition of cystine tellurite Y lamb's blood is ideal for Corynebacterium diphteriae.įor its part, the prepared agar is light amber in color. On the other hand, if antibiotics are added to the medium, it becomes a selective medium. However, for fastidious bacteria it is necessary to supplement this agar with defibrinated blood or warmed blood to increase its enrichment. In this sense, triptein and soy peptone provide these nutrients to microorganisms, thus allowing their full development. Basisįor the proper development of bacteria, the presence of energy supplies is necessary, such as amino acids, vitamins, puric and pyrimidic bases. On the other hand, when combined with antibiotics it is useful to isolate clinically important facultative and strict anaerobic microorganisms from samples with mixed flora.įinally, the composition of trypticasein soy agar and its performance comply with the requirements established by the different pharmacopoeias (European, Japanese and North American). The medium without additional supplements is not recommended for primary cultures, but is very useful for subculturing pure strains and keeping them viable, among other uses.Īlso, this agar serves as a base for the preparation of enriched media such as blood agar, especially when it is required to observe the hemolysis patterns and to mount the optoquine and bacitracin taxa, required in the diagnosis of Streptococcus pneumoniae Y Streptococcus pyogenes respectively. It is composed of triptein, soy peptone, sodium chloride, and agar-agar.ĭue to its high nutritional power, it is ideal for the cultivation of moderately demanding and non-demanding microorganisms. It is designated by the letters TSA for its acronym in English Trypticase Soy Agar. The Trypticasein Soy Agar or Trypticase Soy Agar is a solid, non-selective and nutritious culture medium. Growth control and hemolysis pattern of trypticasein soy agar supplemented with blood.Trypticasein soy agar plates supplemented with warmed blood.Trypticasein soy agar plates supplemented with blood. ![]()
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